| There are two main methods which can be
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| | Polymorphism test takes longer than the
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| used for paternity DNA testing, namely,
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| | PCR test and is a slightly older method
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| PCR (Polymerase Chain Reaction) and RFLP
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| | but can provide more reliable results as
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| (Restriction Fragment Length
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| | each loci presents more information
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| Polymorphism). In this article we are
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| | regarding paternity. It requires a larger
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| going to be looking at these two methods,
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| | sample of DNA and is more likely to use
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| what the difference is and how they work.
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| | blood for the DNA testing but it can also
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| Polymerase Chain Reaction testing usually
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| | be done using a swab from the inner cheek
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| involves taking a swab from the inner
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| | if necessary.
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| cheek for DNA samples. It is a faster
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| | The AABB report from 2004 stated that
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| test than RFLP and usually looks at
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| | there is a decrease in the number of
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| between six and nine loci on the DNA.
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| | laboratories using the RFLP method and an
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| This test however does not provide the
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| | increase in laboratories using the PCR
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| same degree of information as is provided
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| | method of DNA testing with PCR being used
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| by the RFLP test.
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| | in 98.34% of the cases.
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| PCR DNA testing works by "amplifying" the
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| | In conclusion, there are two main methods
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| sample DNA, or taking a small sample of
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| | of paternity DNA testing that are mainly
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| DNA and then multiplying it. This is
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| | used today. These two methods are
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| useful if only a small sample can be
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| | Polymerase Chain Reaction testing and
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| obtained and is also useful for working
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| | Restriction Fragment Length Polymorphism
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| with degraded DNA. When using PCR DNA
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| | testing. PCR testing is used in 98.34% of
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| testing however the laboratory needs to
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| | the DNA testing cases according to the
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| be particularly careful about preventing
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| | 2004 AABB report and seems to be
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| contamination within the sample as the
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| | increasing over the older method of RFLP.
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| amplification process could tend to
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| | PCR is a quicker method of testing and
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| increase the chances of contamination.
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| | multiplies the amount of DNA material so
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| The process of PCR DNA testing involves
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| | that only small samples are needed;
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| heating the DNA, adding primers and then
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| | however this does lead to a risk of
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| cooling it so that it recombines and an
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| | contamination. If you are going for PCR
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| enzyme reads the DNA sequence in order to
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| | testing or ordering a DNA home test kit
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| create multiple copies of the DNA. Visit
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| | you should ensure that they are AABB
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| for more info on genetic testing.
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| | approved and that they offer at least a
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| The Restriction Fragment Length
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| | 99% guarantee and test at least ten loci.
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